Utilizing flow cytometry sorting signal width to enrich for cells positive to endogenous gene integration of fluorescent proteins
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下载链接：
http://flowrepository.org/id/FR-FCM-Z66A
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资源简介：
To efficiently identify nuclear localized protein in heavily mixed populations in order to generate cell lines with properly integrated fluorescent tags after gene knock-in using CRIPSR Cas12.
Conclusion:
By measuring the GFP-W of the cells rather than the standard GFP-A, we could successfully identify nuclear localized protein and generate single cell lines with positive gene integration with relative ease CS&T beads, Accudrop beads sort calibration according to manufacture recommendations
创建时间：
2023-04-01
