Effect of Tramesan on wheat leaves infected with P. nodorum.
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Durum wheat leaves of an Italian commercial variety were sprayed with a solution of 0.38μM Tramesan and after 48 h, inoculated with 105 conidia of P. nodorum; other plants were inoculated with the pathogen but not pre-treated with Tramesan (Infected control). Disease severity was quantified by using Liu’ scale (necrotic spot extension) whereas relative expression of the peroxidase-encoding, PR-9 gene, was calculated using the 2-ΔΔCt method. Fungal growth was assessed by qPCR using P. nodorum specie-specific primers.
创建时间:
2017-08-23



