Suppl dataset 2 CHIPseq bZIP60 & 17|基因表达调控数据集|DNA测序数据集

Chromatin immunoprecipitation analysis was carried out in triplicate to identify the direct transcriptional targets for ZmbZIP60 and ZmbZIP17. GFP tagged forms of the bZIP transcription factors (minus their transmembrane and lumen-facing domains) were transfected into maize seedling mesophyll protoplasts and after incubation nuclei were isolated, chromatin extracted and fragmented by sonication. GFP tagged ZmbZIP60 and ZmbZIP91 were immunoprecipitated with anti-GFP antibody and the coimmunoprecipitated DNA fragments were eluted, used to construct DNA libraries and subject to DNA sequencing. Read sequences were aligned to maize genome version 4. Peaks were identified and their intersection with 500 bp upstream in the promoter regions of the various genes was computed to give a peak score.