broadinstitute/lincs-cell-painting: Full release of LINCS Cell Painting dataset

Summary

This release contains finalized profile and metadata information for the CellProfiler-derived features from the LINCS Cell Painting dataset.

We assayed two batches of data and applied an image analysis pipeline using CellProfiler to segment cells and extract morphology features. Using the CellProfiler output, we applied an image-based profiling pipeline to process the morphology profiles into an analysis-ready form.

In this repository, we provide the full image-based profiling pipeline we used to process the data, as well as most of the intermediate data.

Data counts

Two batches ("2016_04_01_a549_48hr_batch1" and "2017_12_05_Batch2")

159,717,488 unique cells total

2016_04_01_a549_48hr_batch1

52,223 profiles (batch corrected)

10,752 unique perturbations

1,571 unique compounds

1 time point (48 hour)

7 doses

1 cell line (A549)

110,012,425 unique cells

2017_12_05_Batch2

51,447 profiles (batch corrected)

10,368 unique perturbations

349 unique compounds

3 time points (6 hour, 24 hour, 48 hour)

6 doses

3 cell lines (A549, MCF7, U2OS)

49,705,063 unique cells