Intestinal and thymic tuft cell RNA-seq

The goal of the study was to sequence mRNA from tuft cells (identified as CD45-;EpCAM+;IL-25+ using Flare25 reporter mice) in the epithelia of thymus and small intestine. As a control, non-tuft epithelial cells (CD45-;EpCAM+;IL-25-) were also isolated. The data were used to compare tuft cell markers between the small intestine and thymus. Four replicates of tuft cells were sorted from both tissues. As a reference, four replicates of non-tuft cells were also sorted from the small intestine. One tuft replicate from the small intestine was discarded because it failed QC. One non-tuft replicate was also discarded because of failed QC本研究的目标为对胸腺与小肠上皮中的簇细胞（tuft cell，通过Flare25报告基因小鼠鉴定为CD45-;EpCAM+;IL-25+）的mRNA进行测序。作为对照，本研究同时分离了非簇状上皮细胞（CD45-;EpCAM+;IL-25-）。本数据集用于对比小肠与胸腺中簇细胞的标记基因。研究人员从两种组织中均分选出4份簇细胞重复样本；作为参照，同时从小肠中分选出4份非簇细胞重复样本。其中1份小肠来源的簇细胞重复样本因质量控制（QC）未通过被弃用，另有1份非簇细胞重复样本同样因质量控制未通过被弃用。