Olig2 Defines a Unique Subset of Neural Stem Cells That Produce Specific Olfactory Bulb Interneuron Subtypes in the Subventricular Zone of Postnatal and Adult Mice

Distinct neural stem cells reside in different regions of the subventricular zone and generate multiple olfactory bulb interneuron subtypes in the adult mouse brain. This study shows that the basic helix-loop-helix type transcription factor Olig2 defines a ventrally enriched subset of NSCs in the early postnatal and adult SVZ and plays an important role in the subtype specification of olfactory bulb interneurons produced by adult NSCs. E18.5, P14 and adult mouse NSCs were analyzed using 10X single-cell rna-seq成年小鼠大脑的侧脑室下区（subventricular zone, SVZ）不同区域存在独特的神经干细胞（neural stem cells, NSCs），它们可在成体脑内产生多种亚型的嗅球中间神经元（olfactory bulb interneuron）。本研究证实，碱性螺旋-环-螺旋转录因子Olig2（basic helix-loop-helix type transcription factor Olig2）可界定出生后早期及成年SVZ中腹侧富集的NSCs亚群，并在成年NSCs所产生的嗅球中间神经元亚型特化过程中发挥关键调控作用。本研究采用10X单细胞RNA测序（single-cell RNA-seq）技术，对胚胎18.5天（E18.5）、出生后14天（P14）及成年小鼠的NSCs进行了分析。