RNA-sequencing in rat hippocampus following chronic ethanol and withdrawal

The objective of The Center for Alcohol Research in Epigenetics (CARE) is to identify gene regulatory pathways in hippocampus that are altered in response to chronic ethanol administration and withdrawal. Overall design: Adult male rats (6 per group) were used for each experimental group: control diet-fed, chronic ethanol diet-fed and 24h withdrawal following chronic ethanol exposure. Hippocampus tissues were dissected out and immediately frozen. Altogether, there was a total of 18 samples (3 groups x 6 rats). The RNAseq libraries were quantitated by qPCR and the 18 libraries were paired-end sequenced on 11 lanes (Illumina Hiseq 4000). Fastq files generated and demultiplexed with bcl2fastq and adaptor sequences were trimmed from the 3'-end of the reads.