Next Generation Sequencing facilitating quantitative analysis of Wild Type and osbzip47dsRNAi transcriptome

Purpose: The goals of this study is to compare NGS-derived inflorescence transcriptome profiling (RNA-seq) between wild type and transgenic rice line #14 silenced for endogenous OsbZIP47 gene through RNA interference Methods: mRNA profiles of tissue pool from 0.1-0.5 cm inflorscences of wild-type (WT) and osbzip47dsRNAi transgenic line #14 were generated by sequencing, in duplicate, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed at the transcript level with STAR2 aligner followed by featureCounts and edgeR Results: we mapped about 30 million sequence reads per sample to the rice genome (RAP Database) and identified 29532 expressed genes mRNA profiles of tissue pool from 0.1-0.5 cm inflorscences of wild-type (WT) and osbzip47dsRNAi transgenic line #14 were generated by sequencing, in duplicate, using Illumina HiSeq.