Sleep Loss Amplifies the Neuron-Specific Interleukin-1 Receptor Accessory Protein Oncogenic Roles

To gain molecular insights into the roles of neuron-specific interleukin-1 receptor accessory protein (AcPb)-dependent responses to sleep disruption (SD), we utilized RNA-seq to compare gene expression in the brains of wild-type (WT) and AcPb-/- mice, both with and without SD. Overall design: We subjected WT and AcPb-/- mice to sleep disruption (SD) from ZT20-4 using a gentle handling technique (SD-WT [n=4], SD-AcPb [n=4]. Control mice (CTL) from both strains (CTL-WT [n=3], CTL-AcPb-/- [n=5]) were left undisturbed before euthanasia at ZT4. RNA sequencing was performed using total RNA extracted from somatosensory cortex tissues collected post-euthanasia from WT and AcPb-/- mice.