Identification and characterization analysis of the different genes in Rag1-/- mouse injected with CD8+TSCM cells induced by CUS using RNA-seq

Whole RNA-sequencing analysis was performed and analyzed by Lc. Biotech Co. Ltd. (Hangzhou, China). hippocampus tissue from the PBS_WT group, PBS_Rag1-/- group, Ctr CD8+TSCM_Rag1-/- group, and CUS CD8+TSCM_Rag1-/- group were collected in TRIzol. Total RNAs were extracted from Trizol. UMI technology was used to label each sequence fragment with sequence tags, which minimized the interference of duplication caused by PCR amplification on the quantitative accuracy of the transcriptome. RNA sequencing reads were aligned to the mouse genome (GRCh37/hg19) using the software Hisat2 (2.0.4). Transcript abundance was evaluated by calculating fragments per kilo base of exon per million fragments mapped (FPKM). Overall design: Examination of different genes by injection of CD8+TSCM cells in Rag1-/- mouse by RNA-seq.