Differentially regulated genes induced by in vitro generated Mycobacterium tuberculosis cell wall deficient forms. Mycobacterium tuberculosis H37Rv

Transcriptional profile of chemically generated Mycobacterium tuberculosis cell wall deficient forms, comparing untreated control cells (bacillary forms) and cell wall deficient cells. Overall design: Two-condition experiment, MTB-K vs. MTB-Sph cells. Biological replicates: 3 controls, 3 spheroplasts, independently grown and harvested. In controls and spheroplasts, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, five replicates per array.