BIO407 Group6 Widefield images of DFX treated cells

## Study:

This data is part of the practical course BIO407 2025 at the University of Zurich, titled 'Advanced Microscopy: From preparation to data and visualization'.

The effects of Deferasirox (DFX) on mitochondria morphology were examined using different microscopy techniques.

Course: BIO407

group-number:

-1: -Jessica Goetschi

-Alexa Fry

-Luca Vogt

## Study Component:

Job: Widefield Microscopy / High Content Screening

Task: 7

Using a widefield microscope, high-content imaging was performed on Deferasirox treated and untreated cells.

## Biosample:

COS-7 cells

## Specimen:

Four different conditions:

-Control 1: No treatment

-Control 2: 5h DMSO treatment

-Condition 1: 3h 200uM DFX treatment

-Condition 2: 5h 200uM DFX treatment

Cells were fixed with 4% PFA, 0.05% GTA, 0.5% Saponin in PHEM.

Cells were labeled with DAPI, TOMM20-Alexa488, Tubulin-Alexa568, SiR-Actin.

## Image Acquisition:

Images were acquired using a LeicaTHUNDER Imager 3D Live Cell on the Leica DMi8 platform.

Acquired channels:

Channel 1: DAPI, 390nm excitation, 460/80nm emission

Channel 2: Alexa488-TOMM20, 475nm excitation, 535/70nm emission

Channel 3: Alexa568-Tubulin, 555nm excitation, 590/50nm emission

Channel 4: SiR-actin, 635nm excitation, 642/80nm emission

Objectives used: 

10x LEICA HC PL FLUOTAR air objective, 0.32NA

20x LEICA HC PL FLUOTAR L air objective, 0.40NA

40x LEICA HC PL APO CORR air objective, 0.95NA

63x LEICA HC PL APO CS2 oil immersion objective, 1.40NA

## Image Data:

Project Name Convention: [Blockcourse]_[Group]_[Microscope]_[Raw/Processed Image]_[DDMMYYYY]_[Sample]_[Task].lif

Image Name Convention: [Magnification x Immersion]_[Additional Information]_[Task]

Additional Information: 

- RFC: Focusing each channel individually during image acquisition

- Sampling: oversample, nyquist, undersample 

- XXXms_exposuretime: time in ms for exposure time

- automatic_acuiqistion: Automatic Acquistion for screening of different conditions

- Position 1-9: 5h DMSO treatment

- Position 10-18: 3h 200uM DFX treatment

- Position 19-27: 5h 200uM DFX treatment

- Position 28-36: No treatment

BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task1.lif

- 10xDry_task1.5

BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task2.lif

- 10xDRY_RFC_task2.1

- 20xDRY_RFC_task2.2

- 63xOIL_task2.3 

BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task3.lif

- 63xOIL_oversample_task3.1

- 63xOIL_nyquist_task3.1

- 63xOIL_undersample_task3.2

BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task4.lif

- 63xOIL_200ms_exposuretime_task4.1

- 63xOIL_400ms_exposuretime_task4.1

- 63xOIL_500ms_exposuretime_task4.1

BIO407_G6_LeicaThunder_Raw_04032025_S6.1_Task7.lif

- 40xAIR_automatic_acquisition_task7_PositionX