Role of Phosphatases in the Regulation of Mitotic Exit, 2016

In “Role of Phosphatases in the Regulation of Mitotic Exit, 2016” we investigated the regulation of cell division. Tight control of this process is essential, and failure to do so can lead to cancer. For many years, the focus of attention has been laid in a group of proteins known as Cyclin-dependent kinases, which work as an engine to drive cell division. These kinases are able to modify other proteins in order to change their function or activity. However, a different sort of proteins known as protein phosphatases can revert the action of these kinases, and hence can also have a major role in controlling cell division. We centered our studies on one of them: PP2A. We found that it is a central component in the regulation of cell division and differentiation, especially in conditions of nutritional stress.

Five datasets are attached to this project:

Data title: PP2A-Pab1 interacting proteins. Proteomic analysis of protein associated to PP2A-Pab1. Pab1 was tagged with an N-terminal tag that was used to pull it down from fission yeast native extracts. Associated proteins were analyzed by tandem mass spectrometry.

Data title: Phosphoproteomic analysis of yeast extracts in cells depleted of PP2A-Pab1. Phosphoproteomic analysis of proteins whose phosphorylation changes upon depletion of PP2A-Pab1.

Data title: Analysis of RNA expression changes in a pab1 deletion mutant in response to nitrogen starvation. Excel file comparing RNA sequencing data. Comparison of RNA expression in a pab1 deleted strain before and after nitrogen starvation treatment (for 4 h).

Data title: Mating counting of different yeast mutants. Excel file containing mating counting as a mean to determine cell differentiation. Different homotallic yeast strains containing different mutations were analyzed regarding their ability to undergo sexual differentiation.

Data title: Real time PCR analysis of mei2 expression in several mutant strains. Excel file containing real time PCR data of mei2 expression normalized to actin expression. Different yeast strain were grown at permissive or at restrictive temperature and the levels of mei2 expression were analysed by RT-PCR