Examining Pathogenesis in Genome Instability Associated Neurodegenerative Mouse Model [Total_PolII_Chip-seq]

Alterations in RNA Polymerase II kinetics can affect key co-transcriptional processes such as normal splicing and transcript elongation and can influence nonsense mediated decay (Fong et al., 2014; Jonkers and Lis, 2015). Specific types of DNA damage can variably influence RNA Pol II activity. We performed Total RNA Pol ll ChIP-seq to investigate how the chronic DNA damage can affect RNA Pol ll activity in the cerebellum tissues. Chip- sequencing of the cerebellum looking at RNA polymerase II in ATM APTX null and associated control brains.