Genetic mapping of barley genes.
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1Forward (F) and reverse (R) primer; primers highlighted in bold were used for direct sequencing for detection of polymorphisms.
2A/GC = anealing temperature/GC buffer (+ = GC buffer added, − = no GC buffer added).
3GenBank accessions for genomic sequences from OWB-D (D) and OWB-R (R) parental lines are indicated. SNP positions are relative to OWB-D.
4Barley chromosome arm: short (s), long (l). HvCMF5 and HvCMF6b were genetically mapped using presence/absence PCR/agarose gel InDel assays.
5Due to lack of polymorphism in the genomic fragment investigated, HvCO15 was mapped using a SNP in an adjacent predicted gene (HvOs08g42430) located on the same sequence contig (Table S4).
创建时间：
2012-09-24
