Supplemental Material for Nelson and Ambros, 2021

Figure S1 contains sanger sequencing confirming let-7 microRNA sequence in C. macrosperma. Figure S2 contains an expanded view of the C. sulstoni and C. macrosperma genomic regions that are syntenic to the let-7 genomic region in C. elegans. Figure S3 contains the C. afra genomic regions that are syntenic to the let-7 genomic region in C. elegans. Figure S4 contains the developmental growth rates of C. elegans, C. macrosperma, and C. sulstoni. Figure S5 contains lin-4 microRNA expression throughout development in C. elegans, C. macrosperma, and C. sulstoni. Figure S6 contains a lin-41 gene tree in Caenorhabditis species. Figure S7 contains examples of the integration of lin-4 and let-7-family microRNAs in C. elegans and C. sulstoni. Figure S8 contains the predicted minimum free energy hybridizations of let-7-family microRNAs with complementary sites in the lin-41 mRNA 3' UTR in Caenorhabditis species. Table S1 contains let-7-family microRNA sequences in Caenorhabditis species. Table S2 contains homologs of heterochronic genes in C. elegans and C. sulstoni. Table S3 contains lin-41 3' UTR bulge-SM+SUP information in Caenorhabditis species. Table S4 contains lin-41 3' UTR GU-SM+SUP information in Caenorhabditis species. Table S5 contains a list of Caenorhabditis strains used in this study. Table S6 contains a list of oligos used in this study. Table S7 contains information on the C. sulstoni lin-41::GFP allele generated in this study. Table S8 contains the normalized reads used for Figure 3. Table S9 contains the exact timing of larval molts used for Figure S4.