OFCCR SNP-CpG

We profiled the methylation landscape of ~2100 DNA samples (~1100 cases of colorectal cancer and ~1000 controls) from the Ontario Familial Colon Cancer Registry (OFCCR) using the HumanMethylation450 array from Illumina. DNA was extracted from lymphocyte pellets, except for 99 samples for which DNA was extracted from lymphoblastoid cell lines. Samples were genotyped on the Affymetrix Human Mapping 100k and 500k arrays. The aim the study was to evaluate the interplay between genetic variation and methylation, including long-range epigenetic regulation.]]> The cases and controls used in this study were enrolled in phase I of the Ontario Familial Colon Cancer Registry (OFCCR). Probands were selected from incident colorectal cancer cases identified between July 1,1997 and June 30, 2000 from the population-based Ontario Cancer Registry. Controls were recruited through telephone interviews in randomly selected households in Ontario.]]>