Bulk RNA sequencing data of macrophages treated with or without sFRP1

Total RNA of BMDMs was isolated using the Trizol reagent. RNA extracted from non-treated BMDMs was marked as control while RNA from sFRP1-treated BMDMs was marked as experiment group. Then, transcriptome sequencing and analysis were performed at Oebiotech Co. Ltd (Shanghai, China). Differential expression analysis was performed using DESeq2 (1.24.0) analysis. P value<0.05 and fold change>2 were set as the threshold for significantly differential expression. All the differentially expressed genes were named as "all DEGs" in the Mendeley repository and the significantly differential genes were named as "Significant DEGs".

采用Trizol试剂对BMDMs的Total RNA进行提取。来自未处理BMDMs的RNA被标记为对照组，而来自sFRP1处理后的BMDMs的RNA则被标记为实验组。随后，在Oebiotech Co. Ltd（上海，中国）进行了转录组测序与分析。差异表达分析采用DESeq2（1.24.0）进行分析。设定P值小于0.05且倍数变化大于2为显著差异表达的阈值。所有差异表达基因在Mendeley仓库中被命名为“all DEGs”，而显著差异基因则被命名为“Significant DEGs”。