Replication data for "A rewired NADPH-dependent redox shuttle for testing peroxisomal compartmentalization of synthetic metabolic pathways in Komagataella phaffii"

Supplementary files of research article "A rewired NADPH-dependent redox shuttle for testing peroxisomal compartmentalization of synthetic metabolic pathways in Komagataella phaffii". This study describes the establishment of the synthetic malonyl-CoA pathway in the peroxisome of Komagataella phaffii for 3-HP production using renewable carbon sources. Several strains were constructed and tested on 24-deep-well plate cultivations containing Buffered Minimal medium supplemented with the appropriate carbon source: glucose (BMD), glycerol (BMG), methanol (BMM), and oleic acid (BMO). Three independent transformants for each strain were cultivated.

A series of strains representing the cytosolic counterparts of three peroxisomal strains were also cultivated in BMD, BMG, BMM, and BMO. An individual clone for each cytosolic strain was cultivated in triplicate in 24-deep-well plates. Full consumption of the substrates and 3-HP concentrations were checked at the end of the cultures.

The attached files contain a description of the molecular cloning materials and methods, and the raw and processed data obtained from the 24-deep-well plate cultivations.