Genetic diversity and population genetic structure of Dendrocalamus brandisii were revealed based on SLAF-seq

Dendrocalamus brandisii is one of the important bamboo species in Yunnan Province, China, with a wide range of uses and high economic and ecological value. Understanding its genetic diversity and genetic structure characteristics provides a theoretical basis for the conservation, utilization, and genetic improvement of D. brandisii resources. In this study, the Specific-Locus Amplified Fragment Sequencing (SLAF-seq) technique was employed to investigate the genetic diversity of 70 individuals from seven geographical populations in Yunnan Province. The results showed that after quality control and filtering, a total of 514 318 SLAF tags were obtained, including 234 305 polymorphic SLAF tags, resulting in 1 651 130 population SNPs and 375 389 highly consistent SNP loci. The average completeness and heterozygosity of SNPs were 54.91% and 18.78%, respectively, with a homozygosity rate of 81.22%. A phylogenetic tree was constructed for the 70 D. brandisii samples, and the clustering results indicated no apparent regional characteristics among the D. brandisii sources in Yunnan Province.