Analysis of nucleotide sequence in EcN mutants with biofilm formation mutants.

a Mutants in bold were selected for further characterisation (See Fig. 1 and associated text).
b Biofilm formation was calculated as a % of the wild-type level quantified using CV staining assay. Results represent the mean of replicate experiments (n = 3) and figures in parentheses show the standard error of the mean. All mutants shown were significantly altered in biofilm formation compared to the wild-type (P < 0.05).
c BFD—biofilm formation deficient; BFE—biofilm formation enhanced.
d Putative functions of disrupted genes were primarily assigned based on the correlation of sequences flanking mini-Tn5 inserts to the EcN draft genome sequence [28]. ~40 nt directly flanking the transposon were mapped to EcN contigs.
* In cases where EcN genome annotations did not provide a clear indication of function, BlastX searches against the full nr dataset, and/or the conserved domain database were used to infer function, with values in parentheses providing e-value and identity for these searches.
+ indicates genes predicted to be involved in synthesis of surface associated structures, broader aspects of cell envelop biogenesis, or associated with the cell envelop.
Analysis of nucleotide sequence in EcN mutants with biofilm formation mutants.