Transcriptomic analysis of AGS treatment in human mesothelioma

We analysed the molecular effects on gene modulation exerted by aglianico grape seed extract (AGS) in mesothelioma. Previous results allowed to demonstrate that AGS polar extracts determined apoptosis in three different mesothelioma cell lines including one resistant to conventional chemotherapeutic treatment. In addition this treatment affected tumorigenic and invasive properties of these cells We used microarrays to identify molecular pathway deregulated by AGS treatment and involved in apoptosis induction. Human malignant mesothelioma MSTO-211H cells were treated with 350 micrograms/ml of AGS for 48 hours with a double administration at 24 and 48 hours. Cells treated with the vehicle (DMSO) were used controls. RNA extracted from treated and control cells were used for transcriptomic analysis using Clariom S Array