An environment-responsive SCLA-type GRAS protein controls reproductive strategy in Marchantia polymorpha

This dataset contains raw reads from RNA sequencing experiments of 2 week old Marchantia polymorpha plants completed in Hoey et al. (2024). The experiments are completed on two genotypes, WT (Tak-1), and mutant (gras7-1). MpGRAS7 is an SCLA-type GRAS transcriptional regulator in Marchantia polymorpha. These experiments were completed to understand the role of MpGRAS7 in plant stress. There are two experiments: plants harvested after 24 h continuous Far Red (FR) light, and plants harvested after 1 h and 4 h treatment with abscisic acid (ABA). Details of treatment and extraction are as follows. FR-treatment transcriptome: 2-week old Marchantia polymorpha samples, grown on 1/2 MS B5 medium (pH 6.7). Tak-1 and gras7 genotypes were grown on square plates for 2 weeks (14 days) in continuous white light conditions (70 uE m-2 s-1) at 22 degrees Celsius, and transferred to WL supplemented with FR light conditions for 24 hours. Control plants were left in the WL-only chamber for this time period. Plants were flash frozen, with 3-4 individual plants constituting a single sample. ABA-treatment transcriptome: 2-week old Marchantia polymorpha samples, grown on 1/2 MS B5 medium (pH 6.7) in continuous white light conditions (70 uE m-2 s-1) at 22 degrees Celsius. Tak-1 and gras7 genotypes were grown on square plates for 2 weeks in WL-only conditions. Samples were droplet-treated with 100uM ABA (one 20uL drop per lobe of plant). Mock samples were treated with a mock solution (ddH2O supplemented with an equivalent volume of DMSO, the solvent in which the ABA stock was dissolved). Samples were harvested and flash frozen at 1h and 4h post-treatment, with 3-4 plants constituting a single sample. For all samples: RNA was extracted using PureLink(TM) Plant RNA reagent (Thermofisher UK, catalog number: 12322012). RNA was then treated with TURBO(TM) DNase (Thermofisher UK, catalog number: TAM2238) to remove contaminating DNA. Sample concentrations were measured on a Nanodrop, and sent to sequence via a commercial RNASeq provider (Novogene UK).