E. coli arabinose- & rhamnose-responsive promoter library

This study elucidates the binding sites of transcription factor, AraC and RhaS, to identify functional mutations to engineer promoters with desired properties. A diverse library of the arabinose- and rhamnose-inducible promoters, pBAD and pRha, was sorted into bins (B1 to B4) based on GFP expression levels in the presence or absence of their respective inducers in E. coli. The promoter sequences of the pre- and post-sorted expression bins are deposited here.