Molecular roadmap of the healthy stress response in the mouse hippocampus

The acute stress response mobilizes energy to meet situational demands and re-establish homeostasis. Here, we used a brief forced swim exposure to trigger a strong stress response in mice. Using multiomic profiling we characterize the stress-induced molecular events in the dorsal (dHC) and ventral (vHC) hippocampus over time, across molecular scales, and down to the level of cell types and single cells. Examining the effect of acute swim stress (6 min at 18°C) on the transcriptome and translatome of the mouse hippocampus at multiple time-points (all data: Stressome_GeneCounts.txt). This series contains data from multiple experiments investigating this. Following sub-experiments were conducted: TimeSeriesFST (TimeSeriesFST_GeneCounts.txt): Experiment to assess stress induced transcriptional changes in the hippocampus at multiple time points (45min, 1h30min, 2h, 3h, 4h) vs controls in the dorsal (dHC) and ventral hippocampus (vHC). FSTFemale45min4h (SwimFemale45min4h_GeneCounts.txt): Experiment replicating two time points (45min and 4h) from the time-series with female and male mice to investigate sex effects in the vHC. LeftvsRight (LeftvsRight_GeneCounts.txt): Experiment to assess differences in the acutes stress response (45min) between left and right hemisphere in both dHC and vHC. CMVTRAP (CMVTRAP_GeneCounts.txt): Experiment to assess changes in ribosome occupancy (active translation) in a ubiquitous TRAP assay (all cell types) at 45minutes in the vHC in both pre- and post-IP fractions. CAMK2A-TRAP (CAMK2A-TRAP_GeneCounts.txt)/CAMK2A-TRAP-2 (CAMK2A-TRAP_Exp2_GeneCounts.txt): Two experiments to assess translation changes in excitatory neurons after acute stress in the dHC and vHC at 45 min and 1h 30min. VIAAT-TRAP (VIAAT-TRAP_GeneCounts.txt): Experiment to assess translation changes in inhibitory neurons after acute stress in the dHC and vHC at 45min急性应激反应可调动能量以应对情境需求并重建内稳态（homeostasis）。本研究采用短时强迫游泳刺激，在小鼠体内诱发强烈的应激反应。我们通过多组学表征（multiomic profiling），对不同时间节点、不同分子尺度，乃至细胞类型与单细胞层面的背侧海马（dorsal hippocampus, dHC）和腹侧海马（ventral hippocampus, vHC）中应激诱导的分子事件进行了系统刻画。 本研究考察了18℃环境下6分钟急性游泳应激对小鼠海马转录组与翻译组在多个时间点的影响（全部数据集：Stressome_GeneCounts.txt）。本数据集系列包含多项相关实验的数据，具体子实验如下： 1. TimeSeriesFST（TimeSeriesFST_GeneCounts.txt）：该实验旨在评估背侧海马（dHC）与腹侧海马（vHC）在多个时间节点（45min、1.5h、2h、3h、4h）下应激诱导的转录组变化，并与对照组进行比较。 2. FSTFemale45min4h（SwimFemale45min4h_GeneCounts.txt）：该实验复刻了时序实验中的两个时间点（45min与4h），使用雌性与雄性小鼠，以探究腹侧海马（vHC）中的性别差异效应。 3. LeftvsRight（LeftvsRight_GeneCounts.txt）：该实验旨在评估急性应激（45min）下，背侧海马（dHC）与腹侧海马（vHC）的左右半球间应激反应的差异。 4. CMVTRAP（CMVTRAP_GeneCounts.txt）：该实验采用通用TRAP检测（TRAP assay，覆盖所有细胞类型），评估了腹侧海马（vHC）在应激45分钟时，免疫沉淀（IP）前后组分中核糖体占据率（活跃翻译水平）的变化。 5. CAMK2A-TRAP（CAMK2A-TRAP_GeneCounts.txt）/CAMK2A-TRAP-2（CAMK2A-TRAP_Exp2_GeneCounts.txt）：两项实验旨在评估45min与1.5h时，背侧海马（dHC）与腹侧海马（vHC）中兴奋性神经元在急性应激后的翻译组变化。 6. VIAAT-TRAP（VIAAT-TRAP_GeneCounts.txt）：该实验旨在评估45min时，背侧海马（dHC）与腹侧海马（vHC）中抑制性神经元在急性应激后的翻译组变化。