Relative receptor affinities, metabolic and mitogenic potencies, and IR off-rates [% of human insulin].

All assays were performed in at least three independent experiments. Data are means ± SD and presented relative to human insulin. For human insulin, IR assay IC50 values were in the picomolar affinity range, IGF-IR assay IC50 values were in the nanomolar affinity range, rFFC assay EC50 values were in the picomolar range, and mitogenic assay EC50 values were in the nanomolar range (HMECs) and low nanomolar range (L6-hIR). The dissociation rate constant for human insulin was (3.7±0.3×10−2 min−1).