Adoptive Transfer of Vaccine-primed Circulating T cells in Ovarian Cancer

The treatment of recurrent ovarian cancer (OC) patients remains a challenge. We previously showed that recognition of tumor neoantigens in OC1 can be amplified by vaccination using autologous dendritic cells (DCs) pulsed with oxidized whole-tumor lysate2. Here we report that such responses can be further reinvigorated by the subsequent adoptive cell transfer (ACT) of autologous vaccine-primed ex vivo expanded peripheral blood T cells. Restoration of vaccine-induced neoantigen specific T-cell responses and epitope spreading with novel neoantigen reactivities were found post-ACT in patients with clinical benefit, where we further detected circulating tumor (ct)DNA corresponding to immunogenic neopeptides, suggesting reinvigoration of tumor-sculpting immunity.