Quantification of microRNA expression in early-stage oral squamous cell carcinoma

Microarray was used to find out the differentially expressed in tumor sites of early-stage oral squamous cell carcinoma compared with Normal parts. Furthermore, we compared cases of early-stage oral squamous cell carcinoma with lymph node metastasis with cases without lymph node metastasis. The miRNAs obtained may not only serve as predictive biomarkers for lymph node metastasis, but may also be used further to understand disease. Human oral tissues samples were collected from 14 men and 16 women who were diagnosed with early OSCC (clinical Stage I-II) in the tongue at the Oral Surgery Department of Ehime University School of Medicine hospital for a total of 30 patients for 15 years from March 2006 to October 2020. Of these patients, 15 patients had cervical lymph node metastasis.