Second order rate constants for atrazine, melamine and ametryn for the AtzA and TriA variants that comprise the major trajectories between AtzA and TriA and vice versa.

The kcat/KM values (± standard deviation) are shown. The kcat/KM values for the all of the variants shown in Fig. 2 and Fig. 3 can be found in Table S2. The leaving group for each hydrolysis is shown in parentheses next to the name of the substrate. ND = Not determined; BDL = below detection limit. Each variant has been assigned a letter and the identity of the each variant’s direct parent is indicated together with the distinguishing amino acid substitution. The letter assignments correspond to those found in the figures.