snRNA-seq of developing marmoset female and male gonads across development

This study used droplet-based snATAC-seq to profile gene expression of 45,103 nuclei in the developing marmoset female and male gonads across several timepoints during development, from gestational day (GD) 92 until 3 weeks after birth (wo). Embryos were collected via hysterotomy. A specialized veterinarian performed all surgical procedures, including anesthesia and analgesia. Additionally, postnatal samples from newborn and 3weeks-old marmoset were collected following euthanasia. Single-cell barcoding and library preparation were performed using 10x single-cell ATAC reagent kit (v1.0) and a Chromium controller. Libraries were sequenced using paired-reads on Illumina NextSeq 550 and initial data processing was performed using Cellranger ATAC (1.1).