RNA-seq analysis of kidney cortex of proximal tubular epithelial cell specific I-PpoI expressing mice.

For determination of the changes in PTEC gene expression caused by DNA DSBs, RNA-seq analysis was performed. RNA was isolated from the renal cortex from the I-PpoI or WT mice. Total RNA was used to prepare an RNA-seq library using the TruSeq Stranded mRNA LT Sample Prep Kit according to the TruSeq Stranded mRNA Sample Preparation Guide, part #15031047 Rev. E (Illumina, San Diego, CA, USA).