Transcriptome expressed in the mouse suprachiasmatic nucleus

This array set was used to identify the genes that are highly expressed in the mouse suprachiasmatic nucleus (SCN). Because pharmacological inhibition of Gai/o activity with pertussis toxin hampers intercellular synchronization and causes dampened rhythms of the entire SCN, we hypothesized that member(s) of the Regulator of G protein Signaling (RGS) family might contribute to synchronized cellular oscillations in the SCN. To test this hypothesis, we surveyed all known mouse Rgs genes for their expression by using GeneChip and selected the genes that are highly expressed in the SCN for further analysis. 12h light:12h dark cycle entrained animals were released into constant darkess. SCN punches were taken from 10 animals at CT2 and 10 animals at CT14, pooled together, and subjected to DNA microarray analysis. CT2 and CT14 correspond, respectively, to 2 and 14 hours after presumptive onset of daytime under constant darkness.本芯片数据集用于筛选在小鼠视交叉上核（suprachiasmatic nucleus, SCN）中高表达的基因。鉴于使用百日咳毒素（pertussis toxin）对Gai/o蛋白活性进行药理学抑制，会阻碍细胞间同步化并导致整个视交叉上核的节律衰减，我们提出假说：G蛋白信号调节因子（Regulator of G protein Signaling, RGS）家族成员可能参与视交叉上核内细胞振荡的同步化过程。为验证该假说，我们通过基因芯片（GeneChip）技术检测所有已知小鼠Rgs基因的表达水平，并筛选出在视交叉上核中高表达的基因用于后续分析。将以12小时光照:12小时黑暗周期驯化的动物转移至持续黑暗环境中，分别从CT2时间点的10只动物与CT14时间点的10只动物体内获取视交叉上核穿刺样本，将两组样本各自混合后进行DNA微阵列分析。CT2与CT14分别对应持续黑暗环境下预设白昼起始后的2小时与14小时。