Single cell RNA sequencing of large and small airway surface epithelium in newborn cystic fibrosis (CF) and non-CF pigs

The goal of this study was to compare cell composition and gene expression patterns for different cell types in large and small airways of CFTR+/+ (non-CF) and CFTR-/- (CF) pigs. Tissue samples from trachea and small airways (< 2 mm) were collected from newborn pigs, prepared for single cell sequencing via 10X Genomics Chromium controller, and sequenced on Illumina HiSeq4000