MS analyses of mouse VLDL from WT and ApoA5 (Apolipoprotein A-V) knockout mice

The impact of APOA5 on VLDL composition and its potential to alter VLDL metabolism in other ways remains poorly understood. To address this, we investigated the influence of APOA5 on the VLDL proteome. We used VLDL from Apoa5 knockout (KO) and wild-type (WT) mice, for mass spectrometry utilizing two independent laboratories. We identified consistent depletion of 17 proteins involved in lipoprotein metabolism, inflammation, and immune response in Apoa5 KO VLDL, including APOE and serum amyloid A1 (SAA1).