Quantitative acetylome analysis of Fusarium oxysporum

Quantitative proteome and acetylome analyses were conducted by comparing the acetylation levels of the SM to those of the NM. The collected cellular proteins were extracted, digested, and labeled by different TMTs. For acetylation profiling, the pan acetyl-lysine antibody was employed to enrich the acetylated peptides, which were then submitted into mass spectrometer (MS) for LC–MS/MS analysis. For proteome profiling, the peptide fractions were submitted into MS directly.