Dmrt1 maintains testicular immune homeostasis by activating Spry1 and inhibiting NF-κB signaling pathway
收藏科学数据银行2023-02-23 更新2026-04-23 收录
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To explore the potential function of Dmrt1 in spermatogenesis, we constructed a Dmrt1-deficiency mouse model by knockdown Dmrt1 in testicular seminiferous tubule specifically. Total RNAs were extracted from testes using TRIzol Reagent (Invitrogen, cat. NO 15596026).2 μg total RNAs were used for stranded RNA sequencing library preparation. RNA libraries for RNA-seq were prepared using KC-Digital Stranded mRNA Library Prep Kit for Illumina (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer’s instruction. To explore the function of Spry1 in testis, we constructed a mouse model with testicular specific KD of Spry1 through seminiferous tubule transplantation. In order to explore the mechanisms of Dmrt1 in regulating testicular immune homeostasis, we performed ChIP-seq analysis on primary SCs infected with a lentiviral vector expressing FLAG-tagged Dmrt1.
提供机构：
Jinlian Hua
创建时间：
2023-02-20
