Methylation state of latent and proliferative metastatic tumor cells. Methylation state of latent and proliferative metastatic tumor cells

During metastasis, cancer cells travel the circulation to colonise distant sites. Due to the rarity of these events, the immediate cell fate decisions of metastasising tumour cells (mTC) are poorly understood and the role of the endothelium, as dissemination interface, remains elusive. Using a novel combinatorial mTC enrichment approach, we provide a first transcriptional blueprint of the early colonisation process Overall design: Mice were injected intravenously with 4T1 breast cancer tumor cells. 3.5 days post-injection, latent and proliferative tumor cells were extracted from the lungs and subjected to WGBS. Comparative analysis of DNA methylation of latent vs. proliferative metastatic tumor cells.