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ACTIVATION TAGGING PLATFORM FOR MAIZE, AND RESULTANT TAGGED POPULATIONS AND PLANTS

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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Disclosed herein is an activation tagging construct for maize, resulting tagged populations and plants. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and CI) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and CI genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.

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2023-02-12
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