Spatial Transcriptomics Uncovers Brain Region-Specific Transcriptional Changes Associated with Development and Aging

This study design encompasses sample diversity across developmental and aging stages, capturing the effects of age-related changes as a central experimental variable under investigation. Six C57BL/6J mice were analyzed using spatial transcriptomics at three distinct time points: postnatal day 21 (P21), 1215 weeks (adult), and 28 months (aged), with two biological replicates for each age group.本研究设计涵盖发育与衰老阶段的样本多样性，将年龄相关变化的影响作为核心实验研究变量开展探究。本研究采用空间转录组学（spatial transcriptomics）技术，对6只C57BL/6J小鼠在三个不同时间节点进行分析：出生后第21天（P21）、1215周（成年阶段）以及28月龄（衰老阶段），每个年龄组设置两个生物学重复。