Panobinostat effectively increases histone acetylation and alters chromatin accessibility landscape in canine embryonic fibroblasts but does not enhance cellular reprogramming

Robust and reproducible protocols to efficiently reprogram adult canine cells to induced pluripotent stem cells (iPSC) are still elusive. Somatic cell reprogramming requires global chromatin remodeling that is finely orchestrated spatially and temporally. Histone acetylation and deacetylation are key regulators of chromatin condensation, which are mediated by histone acetyltransferases and histone deacetylases (HDACs), respectively. HDAC inhibitors have been used to increase histone acetylation, chromatin accessibility, and somatic cell reprogramming in human and mice cells.Our data demonstrate that the unique epigenetic landscape of canine cells does not make them amenable to cellular reprogramming through the proposed treatment, it provides a rationale for a targeted, canine-specific, reprogramming approach by enhancing the expression of transcription factors