RNA-sequencing of C. elegans long-lived mutants eat-2, ife-2, osm-5 and glp-1

Long-lived genetic mutants from different pathways of lifespan extension were used to determine the extent to which there are common downstream mediators of longevity. We have previously obtained RNA-sequencing data from other long-lived mutants including sod-2, clk-1, isp-1, nuo-6 and daf-2. Gene expression will be compared between these nine long-lived mutants. Overall design: Six biological replicates per genotype. Worms were collected as prefertile young adults from NGM plates grown at 20 or 25 degrees Celsius with OP50 E. coli.