ChIP-seq for CTCF, H3K4me3 and H3K27ac following CTCF RNAi in LNCaP cells and IMR90 cells

This study aimed to ascertain the effect of CTCF RNAi on gene expression, chromatin conformation and the chromatin environment in prostate cancer cell line, LNCaP This study aimed to confirm loss of CTCF sites following CTCF RNAi in IMR90 cells Overall design: We performed ChIP-seq for CTCF, H3K4me3 and H3K27ac following 144hrs of Non-Target or CTCF RNAi in LNCaP cells. We performed ChIP-seq for CTCF following 144hrs of Non-Target or CTCF RNAi in IMR90 cells. Each ChIP-seq was performed in duplicate.