Genome-wide identification of long noncoding RNA and their potential interactors in ISWI mutants

Long non-coding RNA (lncRNA) refers to non-coding RNA transcripts with a length of more than 200 nt. They regulate the expression level of target genes. However, the role of lncRNA in chromatin remodeling needs to be further explored. In this study, we used ISWI mutants (ISWI is a chromatin remodeling factor in Drosophila) to explore the function of lncRNA. Based on the transcriptome of ISWI mutant Drosophila, we analyzed the transcripts and conducted differential expression analysis. In ISWI mutants, the expression changes of lncRNAs were more significant. Our results provide a new perspective for understand the possible regulatory role of lncRNA in chromatin remodeling and identify possible lncRNA target genes in ISWI mutants. Overall design: Compare the global expression of ISWI mutant and wildtype Drosophila.