Genome assembly and genome annotation of Leishmania martiniquensis isolated from a leishmaniasis patient in Thailand

Leishmaniasis is a parasitic disease, caused by Leishmania with worldwide distribution. Leishmania martiniquensis is major cause of autochthonous leishmaniasis in Thailand. For better understanding the genome characteristics of L. martiniquensis, illumina sequencing was used to sequence genomic DNA. Fastq paired-end reads were removed adapters and trimmed with Q30 score using Trimmomatic. Trimmed reads were constructed to contigs and scaffolds using Spades. Augustus gene prediction tool for eukaryotic annotation was used to annotate sequences from L. martiniquensis. Predicted amino acid sequences from Augustus were searched to EggNOG and David gene ontology database for finding gene ontology. In addition, annotated genes that passed the criteria e-value < 10e-10 from blastp against protozoa virulence protein database. From this study, we found 201 candidate potential virulence factor genes that were found in protozoa virulence protein database. However, these genes should be validated in further study.