Enhancers contribute to Hdac3-regulated RNA polymerase recruitment to the promoter-proximal region [PRO-seq]

HDAC3 is known to repress gene transcription, however, whether it directly regulates transcription initiation or elongation is still under debate. We generated a PRO-seq dataset derived from WT and Hdac3 KO mouse livers and used NRSA to explore the functional role of Hdac3 in transcriptional regulation. We reported that the deletion of Hdac3 caused a global accumulation of transcription in promoter-proximal regions and enhancer regions. WT and Hdac3-KO mice liver were extracted on day 14. PRO-seq was performed to study transcriptional changes. Two biological replicates were included for each condition.组蛋白去乙酰化酶3（HDAC3）已知可抑制基因转录，但其是否直接调控转录起始或延伸仍存在争议。我们构建了源自野生型（WT）和Hdac3基因敲除（KO）小鼠肝脏的PRO-seq数据集，并采用NRSA探究了Hdac3在转录调控中的功能作用。我们的研究显示，Hdac3的缺失会导致启动子近端区域与增强子区域出现全局性的转录产物积累。我们于第14天提取野生型与Hdac3基因敲除小鼠的肝脏组织，随后开展PRO-seq测序以探究转录水平的变化。每组设置两个生物学重复样本。