Additional file 1 of Tanshinone IIA attenuates neuroinflammation via inhibiting RAGE/NF-κB signaling pathway in vivo and in vitro

Additional file 1: Figure S1. Demonstration of Aβ1-42 oligomers by dot blot and electron microscopy a Dot blot analysis of the composition of Aβ1-42. 1 uL of Aβ1-42 was applied to a nitrocellulose membrane and probed with rabbit anti-oligomer antibody or with anti-amyloid fibril antibody. b Electron microscopy analysis of the structure of the Aβ1-42 aggregates. 20 uL of Aβ1-42 oligomer preparation was dropped onto a 300-mesh carbon nickelgrid, and after 5 min the solution was removed. Sample was stained for 2 min with phosphotungstic acid. The diameter of preparation is consistent with that of the oligomer diameter distribution.