Data associated with "Amyloid formation of alternatively spliced variants of α-synuclein"

<b>Source data-Aggregation kinetics</b>Figure 2A and S2: Aggregation kinetics of SNCA and spliced variants under high agitation conditionsFigure 3A and S5:<b> </b>Aggregation kinetics of SNCA and spliced variants under low agitation conditionsFigure 3B and S6:<b> </b>Aggregation kinetics of SNCAΔ5 and SNCAΔ3Δ5 at low protein concentrationsFigure 4A and S12: Cross-seeding reactions of soluble SNCA with spliced variant fibrils at a seed concentration of 5 mol%Figure 5A and B and S14:<b> </b>Co-mixing reactions of soluble SNCA with alternatively spliced variant monomerFigure S10: Comparison of aggregation kinetics of SNCA and spliced variants at pH 5Figure S11: Self-seeding reactions of SNCA and spliced variants at seed concentration of 5 mol%Figure S13:<b> </b>Cross-seeding reactions of soluble SNCA with spliced variant fibrils at a seed concentration of 10 mol%<b>Figures and Tables</b>Figure 1B: SDS-PAGE analysis of purified recombinant SNCA and spliced variants.Figure 1C: CD spectra of soluble SNCA and spliced variants.Figure 2B: CD spectra taken post-aggregation of SNCA and spliced variant fibrils.Figure 2C and Figure S3: Representative TEM images of SNCA and spliced variant fibrils formed under high agitation conditions.Figure 3C and Figure S7: Representative TEM images of SNCA and spliced variant fibrils formed under low agitation conditions.Figure 4A: SDS-PAGE analysis of soluble and insoluble fractions post-aggregation.Figure 4B: CD spectra of insoluble fraction of SNCA seeded by SNCAΔ5.Figure 4C and D: Representative TEM image of self-seeded SNCA and SNCA cross-seeded with 5% SNCAΔ5 fibrilsFigure 5A and B: SDS-PAGE analysis of soluble (S) and insoluble (I) fractions after ultracentrifugation post-aggregation of SNCA alone, SNCA co-mixed with 25 and 5 µM SNCAΔ3, SNCAΔ5, and SNCAΔ3Δ5, respectively.Figure 5C: TEM images of SNCA co-mixed with either SNCAΔ5 or SNCAΔ3Δ5Table S1. MS analysis of limited PK-digestion of fibrilsFigure S1. LC traces of purified SNCA and spliced variantsFigure S4. SDS-PAGE analysis of PK-digestion of fibrilsFigure S8. Histogram reporting fibril half pitches of SNCAΔ5 and SNCAΔ3Δ5Figure S9. CD spectra of SNCA and spliced variants post-aggregation under low agitation conditions<br><br><br><br><br><br>