Single Cell RNA-seq of PgrCre/+Srfflox/flox and Srfflox/flox mouse uterus at gestation day 3.5

Single cell RNA-seq analysis demonstrated dramatic changes in adult PgrCre/+Srfflox/flox endometrial cell populations compared to Srf flox/flox controls. Uterine Srf ablation caused myeloid immune cell infiltration, and differential expression analysis demonstrated that PgrCre/+Srfflox/flox stromal fibroblasts downregulated progesterone-regulated genes and growth response genes. Proliferating fibroblast populations shifted from G2-M phase-dominant in controls to S-G2 phase-dominant in PgrCre/+Srfflox/flox uteri. PgrCre/+Srfflox/flox epithelial cells overexpressed estrogenic and innate inflammatory genes. Uterine tissues from two mice staged at gestation day 3.5 (3 days after finding vaginal plug) were pooled for each sample, and 2 samples per genotype (from 4 mice per genotype) were sequenced.