Simplified representation of hallmark gene signature scores across different members of the PC9 cell line family.

Single-cell transcriptomics data from all 8 PC9 cell line family members were subjected to a VISION functional interpretation analysis to calculate scores for single cells from 50 MSigDB hallmark gene signatures. Distributions of scores were calculated for each cell line family member-gene signature pair. Signature score distributions that show larger (↑) or smaller (↓) values compared to other cell line family members are noted. Cell line family members with the largest transcriptomic differences (PC9-MGH, PC9-BR1, DS8, and PC9-VU) were chosen for comparisons. “PC9-VU (no DS8)” includes the parental PC9-VU and DS3, DS6, DS7, and DS9 subline distributions, since they all have a large degree of transcriptomic overlap.