Ligand-dependent hedgehog signaling maintains an undifferentiated, malignant osteosarcoma phenotype

To determine the differentially expreesed genes in osteosarcoma cell lines MG63 and U2OS stimulated with hedgehog ligand (SHH) for 24 hours MG63 and U2OS cells were treated with PBS or 1ug/ml of recombinat human sonic hedgehog for 24 hours. RNA was isolated from the cells and RNAseq performed The results present differentially expressed genes when osteosarcoma cell lines are stimulated with SHH mRNA profile of MG63 and U2OS cell lines treated with PBS or SHH